The Acolyte is an unnamed and minor character. He is depicted wearing a jaguar-skin hood and cape. His first appearance is in attempting to chase a woman down who had presumably stolen something from the temple of the gods.
The Acolyte appears to be a servant of Tzekel-Kan, moving to notify him of the arrival of Miguel and Tulio. He is also in charge of security in El Dorado, ensuring the "commands" of the gods are followed. Later in the movie, the Acolyte assists Tzekel-Kan in gathering the ingredients needed to activate a stone golem to be used by Tzekel-Kan. He is sacrificed by Tzekel-Kan after the mad priest discovers his Acolyte is the final ingredient needed to activate the beast.